Beer-Lambert Law Calculator: A = epsilon l c
How much light a solution absorbs, from its concentration and the length of the light path.
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How this calculator works
Choose which quantity you want to find.
Enter the other values in the units shown.
The calculator gives the answer with the formula, the substituted numbers, and the answer in other units.
Formulas used
- A = \varepsilon l c
- c = \frac{A}{\varepsilon l}
Worked example
With the default numbers in the calculator above:
- The formula, arranged to find the absorbance: absorbance = molar absorptivity x path length x concentration
- Put the values in (every value in its SI unit): absorbance = 15000 x 1 x 5.0e-5
- The answer: absorbance: 0.75
Change the numbers in the calculator to see the full working, every step, for your own case.
A second worked example
A different case, with these numbers: What do you want to find? Concentration; Absorbance (leave it as it is if this is what you are finding) 0.75; Molar absorptivity (leave it as it is if this is what you are finding) 15000 L/mol cm; Path length (leave it as it is if this is what you are finding) 1 cm.
- The formula, arranged to find the concentration: concentration = (absorbance) / (molar absorptivity x path length)
- Put the values in (every value in its SI unit): concentration = (0.75) / (15000 x 1)
- The answer: concentration: 5.0e-5 mol/L
- The same answer in other units: 0.05 mmol/L; 50 umol/L
Common questions
What is the Beer-Lambert law?
The absorbance of a solution is proportional to the concentration of the absorbing substance and to the length of the light path through it.
How do I find the transmittance from the absorbance?
Transmittance = 10 to the power of minus A. An absorbance of 0.75 means 17.8 percent of the light gets through.
Why does the law fail at high concentration?
Molecules interact with each other and stray light in the instrument becomes important, so the graph of absorbance against concentration curves.
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Got a question about this? Ask an engineering question and get a plain-language answer with references.
Further reading: Any chemistry text (spectrophotometry, the Beer-Lambert law).
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